Chemometec NC-250 User Manual

NucleoView™ NC-250™ Software User’s Guide P/N 991-0252 Revision 1.1

991-0252 Rev. 1.1 February 2013 10 12. On the ‘Select Additional Tasks’ window check the desired options and click ‘Next’. Checking the ‘Associate

991-0252 Rev. 1.1 February 2013 11 14. On the ‘Window Security’ window click Install. Optional: To avoid this window during future software upgrad

991-0252 Rev. 1.1 February 2013 12 17. The Install program will now have placed two new icons on your desktop. One is the NucleoCounter® NC-250™ in

991-0252 Rev. 1.1 February 2013 13 - FTDI device driver installed - The camera Due to the intentional and very fast reconfiguration of the camera,

991-0252 Rev. 1.1 February 2013 14 Software Upgrade New software releases are typically distributed via our web page or on USB sticks and are insta

991-0252 Rev. 1.1 February 2013 15 Main Window Menu Line: See the Menu Line section below for a more detailed description of content. Image Tab

991-0252 Rev. 1.1 February 2013 16 Result Tab Page: A detailed overview of the results of the active file is displayed when the result tab page in

991-0252 Rev. 1.1 February 2013 17 Operator: The name or initials of the individual that performs the assay can be entered in the operator input f

991-0252 Rev. 1.1 February 2013 18 View -> Event Logs: Opens a window where log files of all operations made by the user are recorded (See detai

991-0252 Rev. 1.1 February 2013 19 Selecting a Protocol in the Main Window Press the ‘select protocol’ button to open the Select Protocol window:

991-0252 Rev. 1.1 February 2013 2 NucleoView™ NC-250™ P/N 991-0252 (English) Revision 1.1 February, 2013 ChemoMetec

991-0252 Rev. 1.1 February 2013 20 Analysis Results When running a protocol the main results (1) will be displayed on the user interface and det

991-0252 Rev. 1.1 February 2013 21 Post Processing File (*.cmpp)  Holds post processing results for a specific *.cm image file corresponding to

991-0252 Rev. 1.1 February 2013 22 Browse Analysis Results Buttons in the upper right corner launches the file browser to browse images from a

991-0252 Rev. 1.1 February 2013 23  Pan and zoom settings are preserved from the previously selected file. The results for the selected file will

991-0252 Rev. 1.1 February 2013 24 File Browser Select a date, a week, a month or a year on the left side of the File Browser. The right side is

991-0252 Rev. 1.1 February 2013 25 File Properties The file properties window contains three or four tab pages:  General with the file name and

991-0252 Rev. 1.1 February 2013 26 Report Generator The Report Generator may be launched in several ways; from the File Browser window by right-

991-0252 Rev. 1.1 February 2013 27 Image Overlay on the User Interface An image overlay may be enabled with the button in the right bottom cor

991-0252 Rev. 1.1 February 2013 28 Next Run When a run is completed, the main result is displayed at the bottom right of the user interface and

991-0252 Rev. 1.1 February 2013 29 Protocols To perform a particular analysis, you must follow the appropriate protocol. This involves selecting an

991-0252 Rev. 1.1 February 2013 3 Caution! This software must be operated as described in this User’s Guide and documents referred to herein. Pleas

991-0252 Rev. 1.1 February 2013 30 Protocol Adaptation Wizard The Protocol Adaptation Wizard will guide you through adapting different parameter

991-0252 Rev. 1.1 February 2013 31 Plot Manager The Plot Manager is used for evaluation and post processing of cell populations found in one or

991-0252 Rev. 1.1 February 2013 32 Three types of files may be added to Plot Manager with the following effects: 1. Image files: These are image

991-0252 Rev. 1.1 February 2013 33 Right-click in the table offers an option to copy table contents to clipboard which can be directly pasted int

991-0252 Rev. 1.1 February 2013 34 File name: The name of the image file is shown with vertical font in the left side of the row window. Check bo

991-0252 Rev. 1.1 February 2013 35 histogram from another row (blue). 1. Data source indicator: A small colored square in the upper left corner

991-0252 Rev. 1.1 February 2013 36 1. Axis Settings: By clicking on the x-axis it is possible to select type of axis, image channel, parameter typ

991-0252 Rev. 1.1 February 2013 37 Add cells outside marker to image overlay(of shown): Selecting this option will add cells outside the selected

991-0252 Rev. 1.1 February 2013 38 Double-click: To launch the large scatter plot double-click in the plot area. In the large scatter plot you can

991-0252 Rev. 1.1 February 2013 39 4. New Quadrant: Creates a new quadrant. Click in the plot area to add the center point of the quadrant. 5. Se

991-0252 Rev. 1.1 February 2013 4 Table of contents Quick Guide ...

991-0252 Rev. 1.1 February 2013 40 Note: Adding cells to the image overlay is cumulative when it is done from the same plot. When adding cells to

991-0252 Rev. 1.1 February 2013 41 Options The options dialog has the following tab pages:  Folders: The location result file folder can be se

991-0252 Rev. 1.1 February 2013 42 21 CFR Part 11 The U.S. Food and Drug Administration has defined regulations for electronic records and electron

991-0252 Rev. 1.1 February 2013 43 NC-250™ application, and are restricted in their use of the application. Running the NucleoView™ NC-250™ applica

991-0252 Rev. 1.1 February 2013 44 License, Protocol and Documentation Installation Introduction To run a given protocol, the corresponding protoc

991-0252 Rev. 1.1 February 2013 45 Maintenance and Backup NucleoView™ Software Maintenance A two-channel image taken in an A8 slide with NucleoVie

991-0252 Rev. 1.1 February 2013 46 How To How to Get Context Sensitive Help While having focus on a control on a specific window, press F1 (not ap

991-0252 Rev. 1.1 February 2013 47 Keyboard Shortcuts Image + Zoom in - Zoom out Arrow keys Pan up, down, left or right in displayed image Applica

991-0252 Rev. 1.1 February 2013 48 Trouble Shooting Installation The NucleoView™ NC-250™ software or the NucleoCounter® NC-250™ instrument is not

991-0252 Rev. 1.1 February 2013 5 Quick Guide 1. Install the NucleoView™ NC-250™ software and the NucleoCounter® NC-250™ instrument as described i

991-0252 Rev. 1.1 February 2013 6 Installation Guide Installation Overview Important: You must be logged on as system administrator to install the

991-0252 Rev. 1.1 February 2013 7 5. Windows will detect the USB stick and present an AutoPlay window if AutoPlay is enabled. Select the Open fold

991-0252 Rev. 1.1 February 2013 8 8. After a few seconds the ‘Welcome to the NucleoView™ NC-250™ Install Wizard’ window will appear. Click ‘Next’.

991-0252 Rev. 1.1 February 2013 9 10. On the ‘Ready to install NucleoView™ NC-250™’ window you will be given options to install or upgrade the Nucl