Chemometec NC-3000 User Manual

NucleoView NC-3000™ Software User’s Guide P/N 991-3003 Revision 1.0

991-3003 Rev. 1.0 August 2013 10 12. On the ‘Select Additional Tasks’ window check the desired options and click ‘Next’. Checking the ‘Associate C

991-3003 Rev. 1.0 August 2013 11 14. On the ‘Window Security’ window click Install. Optional: To avoid this window during future software upgrades

991-3003 Rev. 1.0 August 2013 12 17. After installation of the NucleoView NC-3000™ software two new shortcuts have been placed on your desktop. One

991-3003 Rev. 1.0 August 2013 13 - A USB hub inside the NucleoCounter® NC-3000™ instrument - FTDI device driver installed - The camera Due to the i

991-3003 Rev. 1.0 August 2013 14 Software Upgrade New software releases are typically distributed via our web page or on USB sticks and are install

991-3003 Rev. 1.0 August 2013 15 Installation Qualification (IQ Test) 1. Start the NucleoView NC-3000™ software by double-clicking the icon on t

991-3003 Rev. 1.0 August 2013 16 Operation Qualification (OQ Test) 1. Start the NucleoView NC-3000™ software by double-clicking the icon on the

991-3003 Rev. 1.0 August 2013 17 Performance Qualification (PQ Test) 1. Check that you have a 912-3003 NC Test Kit 3 (Cassette/Slides, beads). 2.

991-3003 Rev. 1.0 August 2013 18 Main Window Menu Line: See the Menu Line section below for a more detailed description of content. Image Tab P

991-3003 Rev. 1.0 August 2013 19 2. Channel color buttons. Colors assigned to each channel. Click on a channel color field to enable/disable the d

991-3003 Rev. 1.0 August 2013 2 NucleoView NC-3000™ P/N 991-3003 (English) Revision 1.0 August, 2013 ChemoMetec A/S

991-3003 Rev. 1.0 August 2013 20 both NC-Slide A2™ and NC-Slide A8™ types are available for the selected protocol the slide type can be changed by

991-3003 Rev. 1.0 August 2013 21 File -> Exit: Shuts down the program. Tools -> Plot Manager: Opens a new Plot Manager window which can be

991-3003 Rev. 1.0 August 2013 22 Selecting a Protocol Press the ‘select protocol’ button (placed just below the F3 button in the right side of the

991-3003 Rev. 1.0 August 2013 23 Analysis Results When running a protocol the main results (1) will be displayed on the user interface and detai

991-3003 Rev. 1.0 August 2013 24 The location may be changed by the user via menu entry Tools->Options Post Processing File (*.cmpp)  Holds

991-3003 Rev. 1.0 August 2013 25 Browse Analysis Results Buttons In the upper right corner launches the File Browser to browse images from a sp

991-3003 Rev. 1.0 August 2013 26  Pan and zoom settings are preserved from the previously selected file. The extended results for the selected fi

991-3003 Rev. 1.0 August 2013 27 File Browser Select a date, a week, a month or a year on the left side of the File Browser. The right side is the

991-3003 Rev. 1.0 August 2013 28 File Properties The file properties window contains three or four tab pages:  General with the file name and t

991-3003 Rev. 1.0 August 2013 29 Report Generator The Report Generator may be launched in several ways; from the File Browser window by right-cl

991-3003 Rev. 1.0 August 2013 3 Caution! This software must be operated as described in this User’s Guide and documents referred to herein. Please

991-3003 Rev. 1.0 August 2013 30 Image Scaling When the image is presented on the screen, the color intensity (pixel intensity values) is scaled a

991-3003 Rev. 1.0 August 2013 31 Image Overlay An image overlay may be enabled with the button near the right bottom corner of the image. It p

991-3003 Rev. 1.0 August 2013 32 Next Analysis When a run is completed, the main result is displayed at the bottom right of the user interface a

991-3003 Rev. 1.0 August 2013 33 Protocols To perform a particular analysis, the appropriate protocol must be used. This involves selecting and run

991-3003 Rev. 1.0 August 2013 34 Protocol Adaptation Wizard The Protocol Adaptation Wizard will guide you through adapting different parameters

991-3003 Rev. 1.0 August 2013 35 Plot Manager The Plot Manager is used for evaluation and post processing of cell populations found in one or mo

991-3003 Rev. 1.0 August 2013 36 the window is always present (beside the button) and saves the current Plot Manager contents to a *.cmpm file pl

991-3003 Rev. 1.0 August 2013 37 Plot Statistics The Statistics window lists detailed gating statistics for all visible polygons and markers. The

991-3003 Rev. 1.0 August 2013 38 Row number: The row number is shown in the upper left corner. Data source indicator: The data source of the cell

991-3003 Rev. 1.0 August 2013 39  Remove Row: Removes this row from the Plot Manger, re-numbers subsequent rows and moves the subsequent rows up.

991-3003 Rev. 1.0 August 2013 4 Table of contents Quick Guide ...

991-3003 Rev. 1.0 August 2013 40 Large Histogram Plot in Plot Manager The large histogram plot is used to set axis parameters, edit marker sets

991-3003 Rev. 1.0 August 2013 41 6. Cancel: Closes the large plot without saving the changes. OK: Closes the large plot and saves the changes. 7.

991-3003 Rev. 1.0 August 2013 42 population, e.g. all non-single cells, then these are not part of the histogram, and therefore will not be include

991-3003 Rev. 1.0 August 2013 43 Large Scatter Plot in Plot Manager The large scatter plot is used to set axis parameters, add or edit polygons an

991-3003 Rev. 1.0 August 2013 44 2. Gating Setup: Launches the Gate Configuration window, where gating settings can be configured for this plot.

991-3003 Rev. 1.0 August 2013 45  Show Info About Selected Gate: Displays a new window with information of how many cells are outside and inside

991-3003 Rev. 1.0 August 2013 46 Gate configuration:  Disable: This option disables gating for the selected gate.  Include: This option results

991-3003 Rev. 1.0 August 2013 47 3. In Plot Manager, open a new scatter plot by clicking on the scatter plot icon on the left-hand side of the d

991-3003 Rev. 1.0 August 2013 48 Export Data In the menu line File->Export Data cell data may be exported to file formats which are compatible

991-3003 Rev. 1.0 August 2013 49 Options The options dialog has the following tab pages:  Folders: The location of the result file folder can

991-3003 Rev. 1.0 August 2013 5 Quick Guide 1. Install the NucleoView NC-3000™ software and the NucleoCounter® NC-3000™ instrument as described in

991-3003 Rev. 1.0 August 2013 50 21 CFR Part 11 The U.S. Food and Drug Administration has defined regulations for electronic records and electronic

991-3003 Rev. 1.0 August 2013 51 3000™ application, and are restricted in their use of the application. Running the NucleoView NC-3000™ application

991-3003 Rev. 1.0 August 2013 52 License, Protocol and Documentation Installation To run a given protocol, the corresponding protocol-, license- a

991-3003 Rev. 1.0 August 2013 53 Maintenance and Backup NucleoView™ Software Maintenance A two-channel image taken in an A8 slide with NucleoView

991-3003 Rev. 1.0 August 2013 54 How To How to Get Context Sensitive Help While having focus on a control on a specific window, press F1 (not appl

991-3003 Rev. 1.0 August 2013 55 Keyboard Shortcuts Image + Zoom in - Zoom out Arrow keys Pan up, down, left or right in displayed image Applicati

991-3003 Rev. 1.0 August 2013 56 Trouble Shooting Installation The NucleoView NC-3000™ software or the NucleoCounter® NC-3000™ instrument is not i

991-3003 Rev. 1.0 August 2013 57 NucleoCounter® instrument The sample tray is ejected and the instrument is on standby The instrument gives abnor

991-3003 Rev. 1.0 August 2013 6 Installation Guide Installation Overview Important: You must be logged on as system administrator to install the Nu

991-3003 Rev. 1.0 August 2013 7 5. Windows will detect the USB stick and present an AutoPlay window if AutoPlay is enabled. Select the Open folder

991-3003 Rev. 1.0 August 2013 8 8. After a few seconds the ‘Welcome to the NucleoView NC-3000™ Install Wizard’ window will appear. Click ‘Next’.

991-3003 Rev. 1.0 August 2013 9 10. On the ‘Ready to install NucleoView NC-3000™’ window you will be given options to install or upgrade the Nucleo