Chemometec NC-200 User Manual

NucleoView® NC-200™ Software User’s Guide P/N 991-0204 Revision 1.1

991-0204 Rev. 1.1 September 2012 10 12. On the ‘Select Additional Tasks’ window check the desired options and click Next. Checking the ‘Associate

991-0204 Rev. 1.1 September 2012 11 14. On the ‘Window Security’ window click Install. To avoid this window during future software upgrades check t

991-0204 Rev. 1.1 September 2012 12 17. The Install program will now have placed two new icons on your desktop. One is the NucleoCounter® NC-200™ i

991-0204 Rev. 1.1 September 2012 13 3. You will now see a series of pop-up messages from the Windows operating system informing about driver insta

991-0204 Rev. 1.1 September 2012 14 6. When NucleoView detects that the instrument has been disconnected, the icon will again turn red, and you wi

991-0204 Rev. 1.1 September 2012 15 You will also be given the option to browse for and select instrument data stored elsewhere. IMPORTANT: You ma

991-0204 Rev. 1.1 September 2012 16 Main Window Menu Line: See the Menu Line section further below. Image Tab Page: If a file is selected the im

991-0204 Rev. 1.1 September 2012 17 Volume input fields: Located below the image are the relevant volume controls for the selected application. Rig

991-0204 Rev. 1.1 September 2012 18 This button starts the analysis according to the selected application. During the analysis an “Analysis in Pro

991-0204 Rev. 1.1 September 2012 19 Selecting a Protocol in the Main Window Press the select protocol button to open the Select Protocol window:

991-0204 Rev. 1.1 September 2012 2 NucleoView®NC-200™ P/N 991-0204 (English) Revision 1.1 September, 2012 ChemoMete

991-0204 Rev. 1.1 September 2012 20 Analysis Results When running a protocol the main results (1) will be displayed on the user interface and det

991-0204 Rev. 1.1 September 2012 21 Plot Manager File (*.cmpm)  Holds a listing of loaded post processing files (*.cmpm)  Is placed in the PM

991-0204 Rev. 1.1 September 2012 22 Browse Analysis Results Buttons Launches the file browser to browse images from a specified date range (day,

991-0204 Rev. 1.1 September 2012 23 File Browser Select a date, a week, a month or a year on the left side of the File Browser. The right side is

991-0204 Rev. 1.1 September 2012 24 Report Generator The Report Generator may be launched in several ways; from the File Browser window by right

991-0204 Rev. 1.1 September 2012 25 Image Overlay on the User Interface An image overlay may be enabled with the button in the right bottom cor

991-0204 Rev. 1.1 September 2012 26 Next Run When a run is completed, the main result is displayed at the bottom right of the user interface and

991-0204 Rev. 1.1 September 2012 27 Protocols To perform a certain analysis, you must follow the appropriate protocol. This involves selecting and

991-0204 Rev. 1.1 September 2012 28 Protocol Adaptation Wizard The Protocol Adaptation Wizard will guide you through adapting different parameter

991-0204 Rev. 1.1 September 2012 29 Plot Manager The Plot Manager is used for evaluating and the post processing of cell populations found in one

991-0204 Rev. 1.1 September 2012 3 Caution! This software must be operated as described in this User’s Guide and documents referred to herein. Plea

991-0204 Rev. 1.1 September 2012 30 Three types of files may be added to Plot Manager with the following effects: 1. Image files: These are image

991-0204 Rev. 1.1 September 2012 31 Right click also offers an option to copy table contents to clipboard which can be directly pasted into a spr

991-0204 Rev. 1.1 September 2012 32 File name: The name of the image file is shown with vertical font in the left side of the row window. Check b

991-0204 Rev. 1.1 September 2012 33 Data source indicator: A small colored square in the upper left corner indicates the source of the cell popula

991-0204 Rev. 1.1 September 2012 34  Parameter types: The available parameters depend on the protocol used to generate the *.cm file. Gating Se

991-0204 Rev. 1.1 September 2012 35 Add cells outside marker to image overlay: Same as the inside-option (see above), but for cells outside selecte

991-0204 Rev. 1.1 September 2012 36 Large Scatter Plot in Plot Manager The large scatter plot is used to set axis parameters, edit polygons and d

991-0204 Rev. 1.1 September 2012 37  Show Info  Copy to Clipboard  Delete BMP to Clipboard: Copies the large plot to the clipboard to paste

991-0204 Rev. 1.1 September 2012 38 Plot Manager Gate Configuration The table in the ‘Gate Configuration’ window is used to configure settings fo

991-0204 Rev. 1.1 September 2012 39 Options The options dialog has three tab pages:  Folders: The result file folder can be set by the user and

991-0204 Rev. 1.1 September 2012 4 NucleoView® NC-200™...

991-0204 Rev. 1.1 September 2012 40 21 CFR Part 11 The U.S. Food and Drug Administration has defined regulations for electronic records and electro

991-0204 Rev. 1.1 September 2012 41 The NucleoView Admin user have full rights in the NucleoView® NC-200™ application, e.g. the user may switch 21

991-0204 Rev. 1.1 September 2012 42 License, Protocol and Documentation Installation Introduction When NucleoView® NC-200™ performs a certain anal

991-0204 Rev. 1.1 September 2012 43 Maintenance and Backup NucleoView® NC-200™ Software Maintenance A two-channel image taken with NucleoView® NC-

991-0204 Rev. 1.1 September 2012 44 How To How to Get Context Sensitive Help While having focus on a control on a specific window, press the butto

991-0204 Rev. 1.1 September 2012 45 Keyboard Shortcuts Image + Zoom in - Zoom out Arrow keys Pan up, down, left or right in displayed image Applic

991-0204 Rev. 1.1 September 2012 46 Trouble Shooting Installation The NucleoView® NC-200™ software or the NucleoCounter® NC-200™ instrument is no

991-0204 Rev. 1.1 September 2012 47 Cassette Issues No cassette is found Non-valid cassette Flow detection error This may be because either

991-0204 Rev. 1.1 September 2012 5 Quick Guide 1. Install the NucleoView® NC-200™ software and the NucleoCounter® NC-200™ instrument as described

991-0204 Rev. 1.1 September 2012 6 Installation Guide Installation Overview Important: You must be logged on as system administrator to install the

991-0204 Rev. 1.1 September 2012 7 Select the Open folder to view files option. 6. The root directory contents on the USB stick will be shown. D

991-0204 Rev. 1.1 September 2012 8 8. After a few seconds the ‘Welcome to the NucleoView® NC-200™ Install Wizard’ window will appear. Click Next.

991-0204 Rev. 1.1 September 2012 9 10. On the ‘Ready to install NucleoView® NC-200™’ window you will be given options to install or upgrade the Nuc